Collagenase substrate per E. Wünsch

(Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg)
C38H52N10O8Mr 776.9CAS [17011-78-8]

The chromo-peptide phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg serves as substrate for the determination of the collagenase units according to Wünsch (1). Collagenase catalyses the hydrolysis of the peptide, the resulting fragment phenylazobenzyloxycarbonyl-Pro-Leu can be detected spectrophotometrically.
A unit according to Wünsch is defined as the turnover of 1 µmole of the substrate per minute at 25 °C and a pH of 7.1.



Purity (HPLC)
> 97.0 %


References:
  1. Evans, C.H. (1981) Biochem. J. 195, 677-84
  2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
  3. Strauch, L. & Vencelj, H. (1967) Hoppe-Seyler's Z. Physiol. Chem. 348, 465-8
  4. Reil-Dlouha, V. et al. (1976) J. Mol. Biol. 107, 293-305
  5. Nagelschmidt, M. et al. (1979) Biochem. Biophys. Acta 571,105-11

EINECS: 241-086-1WGK: 1HS: 29241900
Storage Temperature: -15 °C to -25 °C

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