3,3'-Diaminobenzidine·4HCl·xH2Oresearch grade

(DAB, Diaminobenzidine tetrahydrochloride)
C12H14N4·4HCl·xH2OMr 360.1 (anhydr.)CAS [868272-85-9]

3,3'-Diaminobenzidine tetrahydrochloride (DAB) is a substrate for peroxidases, especially for horseradish peroxidase (HRP).
DAB is used for histology and ultrahistochemistry (1) and demonstration of ultrastructural peroxidase (2).
It serves as a hydrogen donor in the presence of peroxide in the peroxidase reaction. The oxidized DAB forms an insoluble brown end-product, which can be detected in visible light and does not bleach during long-term storage.
Stock solution: 10 - 20 mg/ml in 50 mM Tris buffer, pH 7.3 (store in aliquots at -20 °C), working solution: 0.5 - 1 mg/ml in buffer (PBS, TBS, pH 7.0 - 7.6) containing 0.01 % hydrogen peroxide.
Vials under argon.

Assay (HPLC)
min. 96.0 %


References:
  1. Hanker, J.S. et al. (1972) Histochemie 30, 201-14
  2. Graham, R.C. & Karnovsky, M.J. (1966) J. Histochem. Cytochem. 14, 291- 302
DANGER
Hazard Statements H341 - H350
Precautions P201 - P280
Reaction P308 +P313
Storage P405

Carc. 1B, Muta. 2EINECS: 231-018-9WGK: 3HS: 29215990
Storage Temperature: +2 °C to +8 °C

Cat.No.
Size
EUR
18865.02
1 g
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H341

Deutsch:

Kann vermutlich genetische Defekte verursachen <Expositionsweg angeben, sofern schlüssig belegt ist, dass diese Gefahr bei keinem anderen Expositionsweg besteht>.

English:

Suspected of causing genetic defects <state route of exposure if it is conclusively proven that no other routes of exposure cause the hazard>.

Français:

Susceptible d'induire des anomalies génétiques <indiquer la voie d'exposition s'il est formellement prouvé qu'aucune autre voie d'exposition ne conduit au même danger>.

Italiano:

Sospettato di provocare alterazioni genetiche <indicare la via di esposizione se è accertato che nessun'altra via di esposizione comporta il medesimo pericolo>.

Español:

Se sospecha que provoca defectos genéticos <Indíquese la vía de exposición si se ha demostrado concluyentemente que el peligro no se produce por ninguna otra vía >.

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H350

Deutsch:

Kann Krebs erzeugen <Expositionsweg angeben, sofern schlüssig belegt ist, dass diese Gefahr bei keinem anderen Expositionsweg besteht>.

English:

May cause cancer <state route of exposure if it is conclusively proven that no other routes of exposure cause the hazard>.

Français:

Peut provoquer le cancer <indiquer la voie d'exposition s'il est formellement prouvé qu'aucune autre voie d'exposition ne conduit au même danger>.

Italiano:

Può provocare il cancro <indicare la via di esposizione se è accertato che nessun'altra via di esposizione comporta il medesimo pericolo>.

Español:

Puede provocar cáncer <Indíquese la vía de exposición si se ha demostrado concluyentemente que el peligro no se produce por ninguna otra vía >.

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P201

Deutsch:

Vor Gebrauch besondere Anweisungen einholen.

English:

Obtain special instructions before use.

Français:

Se procurer les instructions avant utilisation.

Italiano:

Procurarsi istruzioni specifiche prima dell’uso.

Español:

Pedir instrucciones especiales antes del uso.

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P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

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P308 +P313

Deutsch:

BEI Exposition oder falls betroffen: Ärztlichen Rat einholen/ärztliche Hilfe hinzuziehen.

English:

IF exposed or concerned: Get medical advice/attention.

Français:

EN CAS d’exposition prouvée ou suspectée: consulter un médecin.

Italiano:

IN CASO di esposizione o di possibile esposizione, consultare un medico.

Español:

EN CASO DE exposición manifiesta o presunta: Consultar a un médico.

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P405

Deutsch:

Unter Verschluss aufbewahren.

English:

Store locked up.

Français:

Garder sous clef.

Italiano:

Conservare sotto chiave.

Español:

Guardar bajo llave.