Deoxyribonuclease I from Bovine Pancreas min. 3000 Kunitz units/mglyophil.

(DNase I, Deoxyribonucleodepolymerase, Deoxyribonucleate 5'-oligo-nucleotide hydrolase, Desoxyribonuclease, Nuklease, Ribonuklease, )
EC 3.1.21.1Mr 31 000 CAS [9003-98-9]

Deoxyribonuclease I, or DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. It cleaves preferentially double-stranded DNA and in the presence of Mg2+ single stranded DNA.

  • Used to nick DNA as a first step to incorporate labelled bases into DNA
  • Suitable for DNase footprinting
  • Improves yield in primary cell isolation by removing extracellular DNA
  • Decreases viscosity in protein samples by removal of DNA contamination


Unit definition: 1 Kunitz unit catalyzes an increase in absorption of 0.001 at 260 nm per minute at 25 °C, pH 5.0 when acting on highly polymerized calf thymus DNA (1).
Activity: min. 3000 Kunitz units/mg lyophilisate


References:
  1. Kunitz, M. (1950) J. Gen. Physiol. 33, 349-62
  2. Laskowski, M. Sr. (1971) The Enzymes IV, 3rd Ed. (Boyer, P.D.ed.) Acad. Press N.Y. 289-311
  3. Moore, S. (1981) The Enzymes XIV, 3rd Ed. (Boyer, P.D. ed.) Acad. Press N.Y. 281-96
  4. Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (5.83, 10.6-10.12, 15.14, 15.16, 15.27-15.31, 13.24-13.25, 13.28-13.29)

EINECS: 232-667-0WGK: 1HS: 35079090
Storage Temperature: -15 °C to -25 °C

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