Deoxyribonuclease I from Bovine Pancreas min. 3000 Kunitz units/mglyophil.
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EC 188.8.131.52 • Mr 31 000 • CAS [9003-98-9]
Deoxyribonuclease I, or DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. It cleaves preferentially double-stranded DNA and in the presence of Mg2+ single stranded DNA.
- Used to nick DNA as a first step to incorporate labelled bases into DNA
- Suitable for DNase footprinting
- Improves yield in primary cell isolation by removing extracellular DNA
- Decreases viscosity in protein samples by removal of DNA contamination
Unit definition: 1 Kunitz unit catalyzes an increase in absorption of 0.001 at 260 nm per minute at 25 °C, pH 5.0 when acting on highly polymerized calf thymus DNA (1).
Activity: min. 3000 Kunitz units/mg lyophilisate
- Kunitz, M. (1950) J. Gen. Physiol. 33, 349-62
- Laskowski, M. Sr. (1971) The Enzymes IV, 3rd Ed. (Boyer, P.D.ed.) Acad. Press N.Y. 289-311
- Moore, S. (1981) The Enzymes XIV, 3rd Ed. (Boyer, P.D. ed.) Acad. Press N.Y. 281-96
- Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (5.83, 10.6-10.12, 15.14, 15.16, 15.27-15.31, 13.24-13.25, 13.28-13.29)
EINECS: 232-667-0 • WGK: 1 • HS: 35079090
Storage Temperature: -15 °C to -25 °C