Protease Inhibitor Mix HP Plus is an effective protection against proteolytic degradation during affinity purification of recombinant polyHis-tag proteins and for other applications, where metal-chelators should be avoided. The mixture of six protease inhibitors has a broad range of activity against aspartate-, cysteine-, and serine proteases as well as aminopeptidases, Thermolysin and other microbial metalloendoproteases. Protease Inhibitor Mix HP Plus contains AEBSF, Bestatin, E-64, Leupeptin, Pepstatin A, and Phosphoramidon.
Supplied as a kit; each vial is provided with an extra vial of 1 ml DMSO.
The content of 1 vial dissolved in 1 ml DMSO will give a 100-fold concentrate, sufficient for 99 ml extract.