Dithiothreitolanalytical grade
(DTT, Cleland's reagent, Dimercapto-2,3-butanediol)
C4H10O2S2 • Mr 154.25 • CAS [3483-12-3]
Dithiothreitol, as well called DTT or Cleland's reagent, quantitatively
reduces disulfide groups (1) and is effective in sample buffers for reducing
protein disulfide bonds prior to SDS gel electrophoresis (SDS-PAGE). The
reducing thiol agent cleaves disulfide bonds between cysteine residues, thus
totally unfolding proteins to allow a separation according to the molecular
weight. It may also be used in lysis buffer in protein sample preparations. It
is not only less pungent and less toxic than 2-mercaptoethanol, but as well a
seven fold lower concentration of DTT (100 mM) than of 2-mercaptoethanol (5 %
v/v, 700 mM) is needed. Oxidized form of DTT max. 0.5 %. Hygroscopic.
Assay (titr.)
MP
|
min. 99.0 %
40 - 45 °C
|
References:
- Cleland, W.W. (1964) Biochemistry 3, 480-2
EINECS: 222-468-7 •
WGK: 3L •
HS: 29309098
Storage Temperature: +2 °C to +8 °C
*Please use the inquiry form in the right column.
Certificates of Analysis
(Lot.-no. - release date)
Material Safety Datasheets
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New glycosidases such as Endo F3, Endo S and Sialidase |
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Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels |
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New protein standards, e.g. SERVA Triple Color Protein Standard I and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection |
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BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104 electrophoresis tank |
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he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG" |
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Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix" |
SERVA Catalog 2023.pdf
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H302
Deutsch:
Gesundheitsschädlich bei Verschlucken.
English:
Harmful if swallowed.
Français:
Nocif en cas d'ingestion.
Italiano:
Nocivo se ingerito.
Español:
Nocivo en caso de ingestión.
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H315
Deutsch:
Verursacht Hautreizungen.
English:
Causes skin irritation.
Français:
Provoque une irritation cutanée.
Italiano:
Provoca irritazione cutanea.
Español:
Provoca irritación cutánea.
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H319
Deutsch:
Verursacht schwere Augenreizung.
English:
Causes serious eye irritation.
Français:
Provoque une sévère irritation des yeux.
Italiano:
Provoca grave irritazione oculare.
Español:
Provoca irritación ocular grave.
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H335
Deutsch:
Kann die Atemwege reizen.
English:
May cause respiratory irritation.
Français:
Peut irriter les voies respiratoires.
Italiano:
Può irritare le vie respiratorie.
Español:
Puede irritar las vías respiratorias.
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P264
Deutsch:
Nach Gebrauch … gründlich waschen.
English:
Wash … thoroughly after handling.
Français:
Se laver … soigneusement après manipulation.
Italiano:
Lavare accuratamente … dopo l’uso.
Español:
Lavarse … concienzudamente tras la manipulación.
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P280
Deutsch:
Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.
English:
Wear protective gloves/protective clothing/eye protection/face protection.
Français:
Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.
Italiano:
Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
Español:
Llevar guantes/prendas/gafas/máscara de protección.
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P301 +P312
Deutsch:
BEI VERSCHLUCKEN: Bei Unwohlsein GIFTINFORMATIONSZENTRUM oder Arzt
anrufen.
English:
IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.
Français:
EN CAS D’INGESTION: appeler un CENTRE ANTIPOISON ou un médecin en cas de malaise.
Italiano:
IN CASO DI INGESTIONE accompagnata da malessere: contattare un CENTRO ANTIVELENI o un medico.
Español:
EN CASO DE INGESTIÓN: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico si se encuentra mal.
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P302 +P352
Deutsch:
BEI KONTAKT MIT DER HAUT: Mit viel Wasser und Seife waschen.
English:
IF ON SKIN: Wash with plenty of soap and water.
Français:
EN CAS DE CONTACT AVEC LA PEAU: laver abondamment à l’eau et au savon.
Italiano:
IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua e sapone.
Español:
EN CASO DE CONTACTO CON LA PIEL: Lavar con agua y jabón abundantes.
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P304 +P340
Deutsch:
BEI EINATMEN: An die frische Luft bringen und in einer Position ruhigstellen, die das
Atmen erleichtert.
English:
IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
Français:
EN CAS D’INHALATION: transporter la victime à l’extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.
Italiano:
IN CASO DI INALAZIONE: trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
Español:
EN CASO DE INHALACIÓN: Transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.
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P305 +P351 +P338
Deutsch:
BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
English:
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue
