Fibronectin (human)lyophil. analytical grade
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(HFN)
Mr ca. 450 000 • CAS [86088-83-7]
Mr ca. 450 000 • CAS [86088-83-7]
Fibronectin enhances cell-cell and cell-substratum adhesion of transformed cells whereby a significant alteration of cellular morphology towards the normal phenotype is observed (1, 2).
Purified by affinity chromatography. Lyophilized from a solution of 150 mM NaCl, 10 mM sodium phosphate pH 7.4.
Reconstitution: Add 5 ml of warm (37 °C) water or PBS. Incubate at 37 °C for 3 hours, occasionally inverting the vial. Do not vortex. Reconstituted solution contains 5 mg of soluble fibronectin and a minor quantity of insoluble fibronectin aggreagate. Filter slowly through 0.2 µm pre-wetted membrane filter. Avoid repeated freezing and thawing.
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Purity (SDS electrophoresis)
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> 90.0 %
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References:
- Ali, I.U. et al. (1977) Cell 11, 115-26
- Millis, A.J.T. & Hoyle, M. (1978) Nature 271, 668-9
- Review:
Kleinman, H.K. et al. (1987) Anal. Biochem. 166, 1-13
EINECS: 289-149-2 • WGK: - • HS: 35040090
Storage Temperature: +2 °C to +8 °C
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