FICOLL™ 400
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Suited for preparation of continuous and discontinuous density gradients. For stabilization of membrane-bound particles, isolation of lymphocytes. Dialyzable material (including NaCl) max. 1 %. Better osmotic properties than sucrose, it preserves functional and morphological integrity and does not penetrate biological membranes.
FICOLL™ 400 is a neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution. Concentrations up to 50 % (w/v), covering a density range up to 1.2 g/ml, can be obtained without exceeding normal osmolarity.
FICOLL = trademark of GE Healthcare companies.
References:
- Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (B.15, 6.12)
WGK: 1 • HS: 39139000
Storage Temperature: +15 °C to +30 °C