Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway. The enzyme is used to test ketose reductase activity in developing maize endosperm.
In 3.2 M ammonium sulfate; pH 6.0.
1 mg corresponds to approx. 0.2 ml, 10 mg correspond to approx. 2 ml.

Unit definition: 1 U catalyzes the oxidation of 1 µmole glucose-6-phosphate per minute at 25 °C, pH 7.8 in the presence of NADP⁺ (equivalent to the reduction of 1 µmol NADP⁺ per minute) (1).
Extraneous activities: 6-PGDH, PGluM each 0.01 %; hexokinase
max. 0.02 %; glutathione reductase max. 0.05 %; PGI max. 0.002 %; creatine kinase max. 0.001 %.


References:

1. Bergmeyer, H.U. (1983) Methods of Enzymatic Analysis, 3rd Ed.Vol. 2, p. 202-3

EINECS: 232-602-6 • HS: 35079090
Storage Temperature: +2 °C to +8 °C