SERVALight Polaris CL HRP WB Substrate Kit


Highly sensitive enhanced chemiluminescence kit for the detection of immobilized proteins (Western Blot) or immobilized nucleic acids (Southern and Northern Blot) labelled directly with horseradish peroxidase (HRP) or indirectly with HRP-labelled antibodies/streptavidin.

The substrate is readily prepared by mixing component A (luminol/enhancer solution) with component B (stabilized peroxide solution) in a one-to-one ratio.
0.1 ml substrate is sufficient for one cm2 membrane.
· High sensitivity, low picogram limit of detection
· Long light emission for 6 hours
· Primary antibody dilution 1:1000 -1:5000
· Secondary antibody dilution 1:20.000 – 1:100.000
· Detection can be done by film or CCD imaging equipment




HS: 38220000
Storage Temperature: +2 °C to +8 °C

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SERVA Catalog
2020

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Western Blotting -
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New !

SERVA Catalog
2020

In detail you will find the following new products in catalog 2020:

  • • SERVA TAE Clear G Agarose tablets for practical nucleic acid electrophoresis
  • • Novel enzymes such as recombinant PNGases F as lyophilized powder
  • • High-performance protein A/G magnetic agarose beads
  • • New buffers for molecular and cell biology
  • • SERVA BluePower® power supplies running from 300 Volt to 6000 Volt and from 150 mA to 2 A for gel electrophoresis and blotting
  • • Electrophoresis gel and blot automation: SERVA BlueStain is an innovative device for the treatment of automated gel and blot staining
SERVA Catalog 2020.pdf
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Certificates of Analysis
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Western Blotting -
New Brochure!
  • • Molecular Weight Standards
  • • SDS PAGE for Western Blotting
  • • Transfer Membranes and Buffers
  • • Blocking Reagents
  • • Detection Systems
  • • Instruments for Western Blotting
SERVA Brochures: Western Blotting V1806 (pdf)
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