N-(2-Hydroxyethyl)piperazine-N'-2-ethane sulfonic acidanalytical grade, for cell culture
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C8H18N2O4S • Mr 238.3 • CAS [7365-45-9]

Tested for use in tissue culture.
At biological pH, HEPES is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 20 = 7.55). Thus, this Good's buffer is used for a wide range of applications in biological research:
Assay (titr.) A 1 cm/10 % in water 260 nm 280 nm Heavy metals (Pb) pH 10 % in water |
min. 99.0 % max. 0.1 max. 0.08 max. 10 ppm 5.0 - 6.5 |
References:
- Good, N.E. et al. (1966) Biochemistry 5, 467-77
- Shipman jr., Ch. (1969) Proc. Soc. Exp. Biol. Med.130, 305-10
- Vega, C.A. & Bates, R.G. (1976) Anal. Chem. 48,1293-6
EINECS: 230-907-9 • WGK: 1L • HS: 29335995
Storage Temperature: +15 °C to +30 °C