N-(2-Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid ·Na-saltanalytical grade
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C8H17N2O4S·Na • Mr 260.3 • CAS [75277-39-3]

N-(2-Hydroxyethyl) piperazine-N'-2-ethane sulfonic acid sodium salt (HEPES sodium salt) is a buffering substance for biochemistry and molecular biology.
At biological pH, HEPES is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 20 = 7.55). Thus this Good's buffer is widely used in biological solutions and as a component in cell culture media.
Assay (titr.) A 1 cm/10 % in water 260 nm 280 nm pH 10 % in water |
min. 99.0 % max. 0.15 max. 0.1 9.5 - 11.5 |
EINECS: 278-169-7 • WGK: 1 • HS: 29335995
Storage Temperature: +15 °C to +30 °C