Isopropyl-β-D-thiogalactopyranosideresearch grade, dioxane-free

(IPTG, Isopropyl-1-thio-β-D-galactopyranoside)
C9H18O5SMr 238.3CAS [367-93-1]

Analog of galactose, not split by β-galactosidase. Inducer of the lac operon in bacteria. Used in conjunction with X-Gal
(5-bromo-4-chloro-indolyl-β-D-galactoside, cat. no. 15243) for detection of lac+ colonies. Dissolve in H20 to 200 mg/ml, sterilize by filtration and store in aliquots at -20 °C. For detection of transformants, use in final concentration of 0.1 mM.

Assay (HPLC)
[α] 20 °C/D (c=1 % in water)
98.0 - 101.0 %
-28.5 ° to -34.5 °
not detected

  1. Donner, J. et al. (1982) J. Biol. Chem. 257, 14826-9
  2. Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (4.33, 4.37-4.38, 1.8-1.9, 17.12-17.13, B.11)
  3. Ed. Ausubel et al. (1994) Current Protocols in Molecular Biology, Massachusetts General Hospital & Harvard Medical School (1.4.3, 16.2.3, 1.15.1)

EINECS: 206-703-0WGK: 1HS: 29389090
Storage Temperature: -15 °C to -25 °C

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