The assay bases on the eco-friendly fluorescent dye SERVA Purple. The dye reversibly binds to lysine, arginine, and histidine residues in proteins and peptides to yield an intensely red-fluorescent product
(ʎ_Ex 518 nm, ʎ_Em 610 nm). The assay exhibits very low protein binding variation, leading to more accurate protein concentration values.

• Fast and simple - no heating and reduction steps, completed in 1 h

• Compatible with many detergents and reducing agents

• Accurate staining of glyco-, phospho-, hydrophobic proteins and peptides

• Single tube and 96- or 384-well-format for high-thoughput

• Detection limit of 100 ng/ml for peptides and 40 ng/ml for proteins

• Linear quantification over 3 orders of magnitude

• Compatible with downstream applications like 1D- and 2D-PAGE, MS, DIGE-labelling, HPLC