Macerozyme R-10 is suitable for the isolation of plant cells, and can be used
in combination with cellulase »Onozuka R-10« (cat. no. 16419) (1, 2) and with
Cellulase »Onozuka RS« (cat. no. 16420).
Activities: Pectinase: ca. 0.5 U/mg Unit definition: 1
U catalyzes the liberation of 1 µmole of reducing groups from pectic acid per
minute at 25 °C, pH 4.5 calculated as galacturonic acid. Reducing groups
determined with alkaline copper reagent (2). Hemicellulase: ca. 0.25
U/mg Unit definition: 1 U is equivalent to 1 µmole of reducing
groups released from beechwood xylan per hour at 37 °C, pH 5.5, calculated as
xylose. Cellulase: ca. 0.1 U/mg Unit definition: 1 U
catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl
cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper
reagent (3). pH optimum: 3.5 - 7.0 Temperature optimum: 40
- 50 °C.
Yamada, Y. et al. (1972) Agr. Biol. Chem. 36, 1055-9
Barraclough, R. & Ellis, R.J. (1979) Eur. J. Biochem. 94, 165-77
Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
How to find the required certificate of analysis for your product: In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02. Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow. Now select the lot number from the list and download the PDF with a click. Done.
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