(Muramidase, Mucopeptide-glycohydrolase, Mucopeptide N-acetyl-muramoylhydrolase) EC 18.104.22.168 • Mr
ca. 14 400
• CAS [12650-88-3]
Crystalline powder in hydrochloride form. Lysozyme hydrolyzes the β 1 → 4
linkages of the murein between N-acetylmuramic acid and N-acetyl-D-glucosamin,
and degrades the heteroglycan chain to disaccharides. This reaction leads to
cell lysis in most gram positive bacteria e.g. Micrococcus luteus. Lysis can
be prevented if the reaction is performed in an isotonic sucrose medium. Under
these assay conditions protoplasts are produced which no longer have a cell
wall. The lysis of Micrococcus luteus cells is used for the activity testing
of lysozyme. In gram negative bacteria a compact lipopolysaccharide layer on
the exposed murein sacculus efficiently shields them from lysozyme digestion.
Only when the stabilizing Ca2+ ions are removed by treatment with
e.g. EDTA, the murein becomes susceptible to lysozyme. Suitable for hydrolysis
of bacterial cell walls and of proteoglycans (1, 2, 4).
Unit definition: 1 unit catalyzes a decrease in absorption at 450 nm of
0.001 per minute at 25 °C, pH 7.0, in a suspension of Micrococcus luteus cells
(reaction volume 3 ml, light path 1 cm) (3).
Isoelectric point: 10.5 - 11.0
Optimum pH: 9.2
Imoto, T. et al. (1972) The Enzymes VII, 3rd Ed. (Boyer, P.D., ed.) Acad.
Press N.Y. 666-70
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