SD Polymerase is a novel artificial thermostable polymerase providing a strong strand displacement activity. Unlike natural enzymes with strong strand displacement activity as Phi29 or Bst Polymerase, which are active below 68 °C only, SD Polymerase is stable up to 93 °C. This allows running isothermal amplification like LAMP with initial heating-up for increased specificity.

SD Polymerase has 5’ – 3’ polymerase and 5’ – 3’ strand displacement activities. It does not have any exonuclease activity. The enzyme does 3’-A-overhangs.

Also available as non HotStart version.

Features

• Higher yields, speed and efficiency compared to Taq Polymerase

• Suitable for amplification of short (100 bp) and long (20 – 30 kb) DNA fragments

• Suitable for simple (e.g. plasmids) and complex (e.g. genomic DNA) templates

Applications

• Suitable for different PCR methods, also long-range PCR

• PCDR (Polymerase Chain Displacement Reaction)

• Strand displacement like LAMP (Loop-mediated isothermal amplification)