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PNGase F, recombinantlyophilized

(Peptide N-Glycosidase F, Endoglycosidase)
Mr ca. 36.000 •

Recombinant glycosidase from Flavobacterium meningosepticum with faster kinetics and greater activity against native glycoproteins.
The proprietary changes made to PNGase F have been shown to have unique characteristics when compared to other commercially-available sources:

  • Does not need a denaturing step
  • Works on native glycoproteins and serum glycoproteins in only minutes at room temperature
  • Leads to a more complete glycan release as compared to other commercially-available enzymes
  • Especially designed and tested for mass spectrometry imaging of tissue samples
  • Contains a His-tag for easy removal by affinity chromatography

    Catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins.
    Because the enzyme is lyophilized, there is no need for refrigerated transport and storage is at room temperature.
    Concentration after reconstitution with 50 µl H2O dest.: 1000 u/µl (2.0 mg/ml) in 1x PBS



    Unit definition: Achieves complete deglycosylation of 10 µg of RNase B incubated in 1x PBS with 1 µl of PNGase F for 5 - 10 min at 37 °C or room temperature.


    HS: 35079090
    Storage Temperature: +15 °C to +30 °C

    • Cat.No.
    Size
    EUR
    36405.01
    100 µg
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