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Amphipol A8-35

(C6.2H10.3O1.35N0.65N0.35)~ 35 • Mr ca. 8 kDa •

Amphipathic surfactant for maintaining solubilized membrane proteins in detergent-free solutions.
Amphipols are a non-detergent alternative for stabilizing membrane proteins in aqueous solutions. Extracted membrane proteins can be exchanged into these amphipathic polymers, keeping them soluble in detergent-free, aqueous solution while stabilizing them biochemically.
Solubility: up to 20 % in water

Applications

  • Cryo- and electron microscopy
  • Stabilization of native membrane protein structure
  • In vitro functional studies
  • Facilitating membrane protein folding/refolding
  • NMR
  • Immobilization of membrane proteins onto solid surface




    References:
    1. Popot, J.-L- et al. (2003) Cell. Mol. Life Sci. 60, 1559-1574
    2. Gohon et al. (2008) biophys. J. 94, 3523-3537
    3. Popot, J.-L. (2010) Annu. Rev. Biochem. 79, 737-775
    4. Popot, J.-L. et al. (2011) Annu. Rev. Biophys. 40, 379-408
    5. Zoonens, M. & Popot, J.-L. (2014) Membr. Biol. 247, 759-796

    HS: 39069090


    • Cat.No.
    Size
    EUR
    60096.02
    100 mg
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    60096.03
    500 mg
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