Nitro blue tetrazolium chlorideanalytical grade

(NBT, Nitro BT, Nitrotetrazolium blue chloride, Ditetrazolium dye)
C40H30Cl2N10O6Mr 817.7CAS [298-83-9]

The ditetrazolium compound nitro blue tetrazolium chloride, also known as NBT for short, serves as a substrate for the detection of succinate dehydrogenase (1), glucose-6-phosphate dehydrogenase (2) and xanthine oxidase (3). NBT is used in conjunction with BCIP (cat. no. 15247) for the colourimetric detection of alkaline phosphatase labelled molecules in Northern, Southern and Western blotting, in situ hybridisation and immunohistochemistry. When incubated with alkaline phosphatase, the BCIP/NBT substrate produces an insoluble, violet-stained NBT-diformazan precipitate that is easily detected on membranes or tissue sections.
BCIP p-toluidine salt and NBT should be dissolved in dimethylformamide to prepare a stock solution.
For added convenience, ready-to-use BCIP/NBT solutions are offered: SERVAColor BCIP/NBT Blot Solution, cat. no. 15245 and BCIP/NBT Ready-to-use Substrate for Immunohistochemistry and Blotting, cat. no. 15246.
Stock solution for Western blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris; pH 9.5).

Purity
min. 98.0 %


References:
  1. Nachlas, M.M. et al. (1957) J. Histochem. Cytochem. 5, 420-36
  2. Negi, D.S. & Stephens, R.J. (1977) J. Histochem. Cytochem. 25, 149-54
  3. Auscher, C. & Amory, N. (1976) Biomedicine 5, 37-8
WARNING
Hazard Statements H302 - H315 - H319 - H335
Precautions P261 - P280
Reaction P301 +P312 - P302 +P352 - P304 +P340 - P305 +P351 +P338

EINECS: 206-067-4WGK: 1HS: 29339980
Storage Temperature: +2 °C to +8 °C

Product Inquiry
*
*
*
*Please add your address and phone number to the field "Message". Samples according to availability. Equipment demonstration on request in Germany and other countries by appointment.
Your data will be processed electronically to answer your inquiry. By sending your request to serva.de you agree with the Privacy Policy.

News

New !

SERVA Catalog
2021

Watch the SERVA Video!

Certificates of Analysis
now online!

Western Blotting -
New Brochure!

Top
Close window
New !

SERVA Catalog
2021

In detail you will find the following new products in catalog 2021:

  • • New enzymes like dsDNase for highly specific degradation of doubled-stranded DNA
  • • Zymolyase® to produce yeast protoplast or spheroplast
  • • Tergitol™ as an alternative to Triton X-100
  • • Easy Peel 2D HPE™ gels for blotting of horizontal 2D gels
  • • New protein standards, e.g. SERVA Unstained Protein Standard 6.5 - 97 kDa
  • • BlueVertical™ PRiME™ Blot Modul for SERVA’s BV-104 electrophoresis tank
  • • IPG tray with electrode lid for 1st dimension protein separation on 7 cm to 24 cm IPG strips
  • • Special Pharma Edition of SERVA BlueStain automated gel staining device

 

SERVA Catalog 2021 (pdf)
Close window
Watch the SERVA Video!

We are SERVA! Watch to see where we are located and how we are serving scientists worldwide.

SERVA Video

Close window
Certificates of Analysis
now online!

How to find the required certificate of analysis for your product:
In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02.
Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow.
Now select the lot number from the list and download the PDF with a click. Done.

Close window
Western Blotting -
New Brochure!
  • • Molecular Weight Standards
  • • SDS PAGE for Western Blotting
  • • Transfer Membranes and Buffers
  • • Blocking Reagents
  • • Detection Systems
  • • Instruments for Western Blotting
SERVA Brochures: Western Blotting V2104 (pdf)
Close window
SERVA - now on YouTube

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.

Close window

H302

Deutsch:

Gesundheitsschädlich bei Verschlucken.

English:

Harmful if swallowed.

Français:

Nocif en cas d'ingestion.

Italiano:

Nocivo se ingerito.

Español:

Nocivo en caso de ingestión.

Close window

H315

Deutsch:

Verursacht Hautreizungen.

English:

Causes skin irritation.

Français:

Provoque une irritation cutanée.

Italiano:

Provoca irritazione cutanea.

Español:

Provoca irritación cutánea.

Close window

H319

Deutsch:

Verursacht schwere Augenreizung.

English:

Causes serious eye irritation.

Français:

Provoque une sévère irritation des yeux.

Italiano:

Provoca grave irritazione oculare.

Español:

Provoca irritación ocular grave.

Close window

H335

Deutsch:

Kann die Atemwege reizen.

English:

May cause respiratory irritation.

Français:

Peut irriter les voies respiratoires.

Italiano:

Può irritare le vie respiratorie.

Español:

Puede irritar las vías respiratorias.

Close window

P261

Deutsch:

Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden.

English:

Avoid breathing dust/fume/gas/mist/vapours/spray.

Français:

Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/aérosols.

Italiano:

Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.

Español:

Evitar respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.

Close window

P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

Close window

P301 +P312

Deutsch:

BEI VERSCHLUCKEN: Bei Unwohlsein GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.

Français:

EN CAS D’INGESTION: appeler un CENTRE ANTIPOISON ou un médecin en cas de malaise.

Italiano:

IN CASO DI INGESTIONE accompagnata da malessere: contattare un CENTRO ANTIVELENI o un medico.

Español:

EN CASO DE INGESTIÓN: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico si se encuentra mal.

Close window

P302 +P352

Deutsch:

BEI KONTAKT MIT DER HAUT: Mit viel Wasser und Seife waschen.

English:

IF ON SKIN: Wash with plenty of soap and water.

Français:

EN CAS DE CONTACT AVEC LA PEAU: laver abondamment à l’eau et au savon.

Italiano:

IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua e sapone.

Español:

EN CASO DE CONTACTO CON LA PIEL: Lavar con agua y jabón abundantes.

Close window

P304 +P340

Deutsch:

BEI EINATMEN: An die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: transporter la victime à l’extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

Close window

P305 +P351 +P338

Deutsch:

BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

English:

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Français:

EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer

Italiano:

IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Español:

EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.