Peroxidase from horseradish min. 1000 U/mglyophil.

(POD, HRP, Donor: hydrogen-peroxide oxidoreductase)
EC 1.11.1.7Mr ca. 40 000CAS [9003-99-0]

For the determination of peroxide (1). Used as an indicator enzyme in reactions where peroxide is produced (2). For labelling antibodies in ELISA (3, 4). RZ (= A 403/A 275) = 3.0.

Unit definition: 1 U catalyzes the decomposition of 1 µmole hydrogen peroxide per minute at 25 °C, pH 7.0; reaction coupled with phenol- aminoantipyrine (5).
Activity in other units: min. 250 purpurogallin units/mg
(1 purpurogallin unit catalyzes the oxidation of 1 mg pyrogallol to purpurogallin in 20 seconds at 20 °C and pH 6.0. The purpurogallin is extracted and determined spectrophotometrically at 420 nm (6). 1 mg purpurogallin requires 13.5 µmole peroxide, 1 purpurogallin unit corresponds to the decomposition of 12 µmoles peroxide at 25 °C.)


References:
  1. Meiattini, F. (1985) Methods of Enzymatic Analysis, (Bergmeyer, H.U., ed.) 3rd Ed. vol. 7, p. 566-71
  2. Bergmeyer, H.U. (1983) Methods of Enzymatic Analysis, 3rd Ed. vol. 2, p. 267-8
  3. Johnson Jr., R.B. (1980) J. Immunoassay 1, 27-37
  4. Harlow & Lane (1988) Antibodies, Cold Spring Harbor Laboratory Press, p. 349
  5. Gallati, H. (1977) J. Clin. Chem. Clin. Biochem. 15, 699-703
  6. Polis, B.D. & Shmukler, H.W. (1963) J. Biol. Chem. 201, 457-500
DANGER
Hazard Statements H334
Precautions P261 - P285
Reaction P304 +P341 - P342 +P311

EINECS: 232-668-6HS: 35079090
Storage Temperature: +2 °C to +8 °C

Product Inquiry
*
*
*
*Please add your address and phone number to the field "Message". Samples according to availability. Equipment demonstration on request in Germany and other countries by appointment.
Your data will be processed electronically to answer your inquiry. By sending your request to serva.de you agree with the Privacy Policy.

News

New !

SERVA Catalog
2020

Watch the SERVA Video!

Certificates of Analysis
now online!

Western Blotting -
New Brochure!

Top
Close window
New !

SERVA Catalog
2020

In detail you will find the following new products in catalog 2020:

  • • SERVA TAE Clear G Agarose tablets for practical nucleic acid electrophoresis
  • • Novel enzymes such as recombinant PNGases F as lyophilized powder
  • • High-performance protein A/G magnetic agarose beads
  • • New buffers for molecular and cell biology
  • • SERVA BluePower® power supplies running from 300 Volt to 6000 Volt and from 150 mA to 2 A for gel electrophoresis and blotting
  • • Electrophoresis gel and blot automation: SERVA BlueStain is an innovative device for the treatment of automated gel and blot staining
SERVA Catalog 2020.pdf
Close window
Watch the SERVA Video!

We are SERVA! Watch to see where we are located and how we are serving scientists worldwide.

SERVA Video

Close window
Certificates of Analysis
now online!

How to find the required certificate of analysis for your product:
In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02.
Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow.
Now select the lot number from the list and download the PDF with a click. Done.

Close window
Western Blotting -
New Brochure!
  • • Molecular Weight Standards
  • • SDS PAGE for Western Blotting
  • • Transfer Membranes and Buffers
  • • Blocking Reagents
  • • Detection Systems
  • • Instruments for Western Blotting
SERVA Brochures: Western Blotting V1806 (pdf)
Close window
SERVA - now on YouTube

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.

Close window

H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

Close window

P261

Deutsch:

Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden.

English:

Avoid breathing dust/fume/gas/mist/vapours/spray.

Français:

Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/aérosols.

Italiano:

Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.

Español:

Evitar respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.

Close window

P285

Deutsch:

Bei unzureichender Belüftung Atemschutz tragen.

English:

In case of inadequate ventilation wear respiratory protection.

Français:

Lorsque la ventilation du local est insuffisante, porter un équipement de protection respiratoire.

Italiano:

In caso di ventilazione insufficiente utilizzare un apparecchio respiratorio.

Español:

En caso de ventilación insuficiente, llevar equipo de protección respiratoria.

Close window

P304 +P341

Deutsch:

BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

Close window

P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.