Polysorbate 80 VGPh. Eur., USP/NF
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(Montanox® 80, Tween® 80, Polyoxyethylene sorbitane monooleate, n ca. 20)
Mr ca. 1300 • CAS [9005-65-6]
Mr ca. 1300 • CAS [9005-65-6]
Polysorbate 80, as well known as Tween® 80, is a non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines and as a stabilizer and emulsifier.
The fatty acids of this detergent are of vegetable origin.
|
HLB CMC d25 °C Acid number Hydroxyl number Saponification number Peroxide value Heavy metals (Pb) |
15.0 1 x 10-5 mol/l 1.06 - 1.09 max. 2.0 mg/KOH/g 65 - 80 mg/KOH/g 45 - 55 mg/KOH/g max. 5 meq/kg max. 10 ppm |
References:
- Sato, M. et al. (1989) Int. J. Biochem. 21, 751-4
- Masaki, S. et al. (1990) Microbiol. Immunol. 34, 653-63
- Okuno, S. & Fujisawa, H. (1990) Biochim. Biophys. Acta 1038, 204-8
EINECS: 500-019-9 • WGK: 1L • HS: 34024200
Storage Temperature: +15 °C to +30 °C
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