Tested for preparation of staining solution for polyacrylamide gel electrophoresis.
Triphenylmethane dye used in protein gel electrophoresis for detection of proteins and with the Bradford method to determine protein concentration. SERVA Blue G is differentiated from SERVA Blue R by the addition of two methyl groups and the slightly greenish tint to its blue color. The dye may exist as a cation (red form) at a pH below 0 with an absorbance peak at 470 nm, an anion (blue form) at a pH above 2 with an absorbance peak at 595 nm, and a neutral, green form at a pH around 1 with an absorbance peak around 650 nm. The blue, anionic form is what binds with amino acid residues, such as arginine or aromatics, to form a stable complex.