SERVA Blue G
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(Acid Blue 90, COOMASSIE® Brilliant Blue G-250, Xylene Brilliant Cyanine G)
C.I.42655 • C47H48N3O7S2·Na • Mr 854.0 • CAS [6104-58-1]
C.I.42655 • C47H48N3O7S2·Na • Mr 854.0 • CAS [6104-58-1]
Tested for preparation of staining solution for polyacrylamide gel
electrophoresis.
Triphenylmethane dye used in protein gel electrophoresis
for detection of proteins and with the Bradford method to determine protein
concentration. SERVA Blue G is differentiated from SERVA Blue R by the
addition of two methyl groups and the slightly greenish tint to its blue
color. The dye may exist as a cation (red form) at a pH below 0 with an
absorbance peak at 470 nm, an anion (blue form) at a pH above 2 with an
absorbance peak at 595 nm, and a neutral, green form at a pH around 1 with an
absorbance peak around 650 nm. The blue, anionic form is what binds with amino
acid residues, such as arginine or aromatics, to form a stable complex.
|
λ max. (0.001 % pH 7) A 1 cm/λ max./1 % pH 7 |
580 - 590 nm min. 450 |
EINECS: 228-058-4 • WGK: 2L • HS: 32041200
Storage Temperature: +15 °C to +30 °C
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