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Tris(hydroxymethyl)aminomethaneanalytical grade, USP

(TRIS, THAM, Trometamol, Tromethamine, Amino-2-(hydroxymethyl)-1,3-propanediol)
C4H11NO3 • Mr 121.1 • CAS [77-86-1]

Tris(hydroxymethyl)aminomethane (Tris) of ultrapure buffer quality with very low UV absorption, suitable for biological, enzymatic and pharmaceutical research.
With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5.
It is used as a component in buffer solutions in numerous applications:
  • Assays in molecular biology and cytology
  • Extraction of proteins or nucleic acids from cells
  • In situ hybridisation procedures
  • Sample and running buffers for SDS-PAGE
  • TAE- and TBE buffers for agarose gel electrophoresis
  • Transfer buffer for Western Blotting
  • Washing buffers for immunoassays

    Assay (titr.)
    A 1 cm/10 % in water
    235 nm
    260 nm
    280 nm
    430 nm
    Heavy metals (Pb)
    pH (5 % in water)

    		 min. 99.9 %

    max. 0.60
    max. 0.15
    max. 0.15
    max. 0.07
    max. 10 ppm
    10.0 - 11.5


    EINECS: 201-064-4 • WGK: 2L • HS: 29221900
    Storage Temperature: +15 °C to +30 °C

    • Cat.No.
    Size
    EUR
    37180.02
    100 g
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    37180.03
    500 g
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    37180.05
    1 kg
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    37180.04
    2.5 kg
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