Tris(hydroxymethyl)methylglycine (Tricine) is a zwitterionic Good's buffer used in biochemistry and molecular biology. The buffer is part of the Tris buffer family and has a useful pH range of 7.4 – 8.8, pKa 20 = 8.15 (1).
Tricine is optimal to separate peptides that are in the molecular weight range of 1 to 100 kDa in SDS-PAGE. It is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography. It is the best buffer for ATP assays involving firefly luciferase and is a scavenger of hydroxyl radicals. Tricine forms a complex with Cu(II) and is therefore used in the Lowry protein assay. The buffer is a non-toxic substitute for barbital (2).

Assay (titr.) A 1 cm/0.1 M in water 260 nm 280 nm pH 10 % in water Heavy metals (Pb) Iron (Fe)

min. 99.0 % max. 0.04 max. 0.02 4.6 - 5.2 max. 5 ppm max. 5 ppm

References:

1. Good, N.E. et al. (1966) Biochemistry 5, 467-77
2. Monthony, J.F. et al. (1978) Clin. Chem. 24, 1825-7

EINECS: 227-193-6 • WGK: 1 • HS: 29225000
Storage Temperature: +15 °C to +30 °C