Brij® 35

Brij 35, an alkyl polyethoxylate, is a mild surfactant suitable for the isolation of functional membrane complexes.

This non-ionic surfactant has been successfully employed in the crystallization of the integral membrane protein cytochrome c oxidase.

Brij 35 can also be used as an additive or modifier in chromatography. It is effective in eluting strongly adsorbed proteins in thiophilic interaction chromatography.

Brij 35 markedly enhances the fluorescence of various dyes and compounds with biochemical or pharmaceutical importance. It is also applied as additive in enzyme assays and in chemiluminescence analysis. 

Brij is a registered trademark of the CRODA International Plc.


Brij L23-PA-(MV), Macrogol Lauryl Ether 20-23, Polyoxyl Lauryl Ether 20-23, C12E23, LAURETH-23, PEG (23) dodecyl ether, PEG (23) lauryl ether

CAS registry number: (9002-92-0)

Relative molecular mass (M): ca. 1200

Classification: non-ionic surfactant

Literature specification:
• Critical micellar concentration (CMC): 0.09 mM
• Aggregation number (Na): 40
• Hydrophilic-lipophilic balance (HLB): 16.9



Solubilization and Characterization of Enzymes

Yoshikawa, S., Tera, T., Takahashi, Y., Tsukihara, T., Caughey, W.S. (1988) Crystalline cytochrome c oxidase of bovine heart mitochondrial membrane: composition and x-ray diffraction studies. Proc. Natl. Acad. Sci. U.S.A. 85, 1354-8.

Endo, S. et al. (1988) Purification and characterization of a substance P-degrading endopeptidase from rat brain. J. Biochem. 104, 999-1006.

Hammer, M.F. et al. (1995) Assessing modulation of stromal and thylakoid light-harvesting complex-II phosphatise activities with phosphopeptide substrates. Photosynth. Res. 44, 107-15.

Jimenez-Cervantes, J. et al. (1995) Effects of detergents and endogenous lipids on the activity and properties of tyrosinase and its related proteins. Biochim. Biophys. Acta 1243, 421-30.

Murphy, A.S. et al. (2002) Identification, purification, and molecular cloning of N-1-naphthylphthalimide acid-binding plasma membrane-associated aminopeptidases from Arabidopsis. Plant Physiol. 128, 935-50.

Tobimatsu, T. et al. (2005) The N-terminal regions of ß and g subunits lower the solubility of adenosylcobalamin-dependent diol dehydratase. Biosci. Biotechnol. Biochem. 69, 455-62.

Use in Enzyme Assays

Frost, D.J. et al. (1994) Characterization of (1,3)-ß-glucan synthase in Candida albicans: microsomal assay from the yeast or mycelial morphological forms and a permeabilized whole cell assay. Microbiology 140, 2239-46.

Nagy, L. et al. (1996) Role of cysteine proteases and protease inhibitors in gastric mucosal damage induced by ethanol or ammonia in the rat. J. Clin. Invest. 98, 1047-54.

Das, A. et al. (1999) Retinal neovascularization is suppressed with a matrix metalloproteinase inhibitor. Arch. Ophthalmol. 117, 498-503.

Jones, A. et al. (2002) Colistin stimulates the activity of neutrophil elastase and Pseudomonas aeruginosa elastase. Eur. Respiratory J. 19, 1136-41.

Dietrich, H.R.C. et al. (2004) Nanoarrays: a method for performing enzymatic assays. Anal. Chem. 76, 4112-7.

de Kleijn, D.P.V. et al (2004) Furin and membrane type-1 metalloproteinase mRNA levels and activation of metalloproteinase-2 are associated with arterial remodelling. FEBS Letters 501, 37-41.

Park, H.I. et al. (2010) Effects of detergents on catalytic activity of human endometase/matrylysin-2, a putative cancer biomarker. Anal. Biochem. 396, 262-8.

Solubilization and Characterization of Various Proteins/Protein Complexes

Ikeda, S, Tsutsui, K., Hatsushika, M.,Watanabe, S.a. Oda, T. (1989) Rapid purification of squirrel monkey retrovirus-H major gag protein by high performance liquid chromatogra­phy. Acta Med. Okayama 43, 127-9.

Cheong, J.J. et al. (1993) Solubilization of functional plasma membrane-localized hepta-[beta]-glucoside elicitor-binding proteins from soybean. Plant Physiol. 103, 1173-82.

Hemler, M.E. (2003) Tetraspanin proteins mediate cellular penetration, invasion, and fusion events and define a novel type of membrane microdomain. Annu. Rev. Cell Develop. Biol. 19397-422.

Klammt, C. et al. (2005) Evaluation of detergents for the soluble expression of a-helical and ß-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J. 272, 6024-38.

Liguori, L. et al. (2007) Production of membrane proteins using expression systems. Expert Rev. Proteomics 4, 79-90.

Schwarz, D. et al. (2007) Preparative scale cell-free expression systems: New tools for the large scale preparation of integral membrane proteins for functional and structural studies. Methods 1, 355-69

Chemiluminescence Analysis/Fluorometry

Javier, B.F., Luis, G.I., Diequez, C., Weeks, I. A. Woodhead, J.S..(1988) Effect of surfactants on the intensity of chemiluminescence emission from acrinidium ester labeled proteins. J. Bio­lumin. Chemilumin. 2, 121-8.

Aiken, J.H., Huie a. C. W. (1991) Detection of bilirubin using surfactant fluorescence enhancement and visible laser fluorometry. Anal. Lett. 24, 167-80.

Ramos-Lledó, P. et al. (2001) Determination of vitamin A and E in milk samples by fluorescence in micellar media. Fresenius J. Anal. Chem. 369, 91-95.

Ahmad, E. et al. (2007) The minimal structural requirement of concanavalin A that retains its functional aspects. J. Biochem. 142, 307-15.

Bandyopadhyay, P. a. Ghosh, A.K. (2010) Reversible fluorescence quenching by micelle selective benzophenone-induced interactions between brij micelles and polyacrylic acids: implications for chemical sensors. J. Phys. Chem. 114, 11462-7.

Chromatographic  Separations

Borgerding, M.F., Hinze, W.L., Stafford, L.D., Fulp G.W. Jr. a. Hamlin, W.C. Jr. (1989) Investigations of stationary phase modification by the mobile phase surfactant in micellar liquid chromatography. Anal. Chem. 61, 1353-8.

EI Rassi, Z. (1989) High performance liquid chromatography of proteins with silica-bound sulfone-thioether functions. BioChromatogra­phy 4, 184-90.

Quian, Y.-W. et al. (1998) Purification and cloning of a protein kinase that phosphorylates and activates the polo-like kinase Plx1. Science 282, 1701-4.

Morimoto, N. et al. (2010) Dual biosynthesis pathway for longer-chain polyamines in the hyperthermophilic archaeon Thermococcus kodakarensis. J. Bacteriol. 192, 4991-5001.

Further Applications

Turner, L. et al. (2000) Real-time imaging of fluorescent flagellar filaments. J. Bacteriol. 182, 2793-2801.

Garcia, J.M. et al. (2001) Influence of the non-ionic surfactant Brij 35 on the bioavailability of solid and sorbed dibenzofuran. Environ. Sci. Technol. 35, 2033-9.

Staats, H. F. et al. (2010) Development of a bead immunoassay to measure Vi polysaccharide-specific serum IgG after vaccination with Salmonella enterica serovar Typhi Vi polysaccharide. Clin. Vaccine Immunol. 17, 412-9.

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