Coomassie® staining

Staining with SERVA Blue R

Reagents and Solutions

Stock solution 1 0.2 % SERVA Blue R (cat. no. 35051) in 90 % (v/v) ethanol (cat. no. 11093) (Solve 100 mg SERVA Blue R in 50 ml ethanol)
Stock solution 2 20 % (v/v) acetic acid
Destainer 20 % (v/v) ehanol, 5 % (v/v) acetic acid, 1 % (w/v) glycerol (cat. no. 23176
Preservation solution 30 % (v/v) ethanol, 5 % (w/v) glycerol

 

Protocol

Perform all fixing and staining work on a shaker at moderate speed (50 rev/min). The specified times apply to incubation at room temperature. Shorter staining and destaining times can be achieved by increasing the temperature.

Fixation/Staining Fixation and staining are done in one step. Stock solution 1 and 2 are mixed in equal parts and the gel is incubated for 30 min in the solution. (Staining solution can be re-used for 2 - 3 times)
Destainer Rinse gel after staining for 1 minute with dist. water and incubate for 2 x 60 minutes in destainer. If back-ground is not clear enough, destain gel for 20 - 30 minutes in 40 % ethanol/10 % acetic acid/2 % glycerol.
Presevation Incubate gel over night in preservation solution. The gel can then be dried in a drying frame.

 

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