Coomassie® staining
Staining with SERVA Blue R
Reagents and Solutions
| Stock solution 1 | 0.2 % SERVA Blue R (cat. no. 35051) in 90 % (v/v) ethanol (cat. no. 11093) (Solve 100 mg SERVA Blue R in 50 ml ethanol) |
| Stock solution 2 | 20 % (v/v) acetic acid |
| Destainer | 20 % (v/v) ehanol, 5 % (v/v) acetic acid, 1 % (w/v) glycerol (cat. no. 23176) |
| Preservation solution | 30 % (v/v) ethanol, 5 % (w/v) glycerol |
Protocol
Perform all fixing and staining work on a shaker at moderate speed (50 rev/min). The specified times apply to incubation at room temperature. Shorter staining and destaining times can be achieved by increasing the temperature.
| Fixation/Staining | Fixation and staining are done in one step. Stock solution 1 and 2 are mixed in equal parts and the gel is incubated for 30 min in the solution. (Staining solution can be re-used for 2 - 3 times) |
| Destainer | Rinse gel after staining for 1 minute with dist. water and incubate for 2 x 60 minutes in destainer. If back-ground is not clear enough, destain gel for 20 - 30 minutes in 40 % ethanol/10 % acetic acid/2 % glycerol. |
| Presevation | Incubate gel over night in preservation solution. The gel can then be dried in a drying frame. |
