Coomassie® staining

Staining with SERVA Blue R

Reagents and Solutions

Stock solution 1 0.2 % SERVA Blue R (cat. no. 35051) in 90 % (v/v) ethanol (cat. no. 11093) (Solve 100 mg SERVA Blue R in 50 ml ethanol)
Stock solution 2 20 % (v/v) acetic acid
Destainer 20 % (v/v) ehanol, 5 % (v/v) acetic acid, 1 % (w/v) glycerol (cat. no. 23176
Preservation solution 30 % (v/v) ethanol, 5 % (w/v) glycerol

 

Protocol

Perform all fixing and staining work on a shaker at moderate speed (50 rev/min). The specified times apply to incubation at room temperature. Shorter staining and destaining times can be achieved by increasing the temperature.

Fixation/Staining Fixation and staining are done in one step. Stock solution 1 and 2 are mixed in equal parts and the gel is incubated for 30 min in the solution. (Staining solution can be re-used for 2 - 3 times)
Destainer Rinse gel after staining for 1 minute with dist. water and incubate for 2 x 60 minutes in destainer. If back-ground is not clear enough, destain gel for 20 - 30 minutes in 40 % ethanol/10 % acetic acid/2 % glycerol.
Presevation Incubate gel over night in preservation solution. The gel can then be dried in a drying frame.

 


Top
Close window
SERVA Catalog
2023

In detail you will find the following new products in catalog 2022:

New glycosidases such as Endo F3, Endo S and Sialidase
Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"
SERVA Catalog 2023.pdf
Close window
Electrophoresis by SERVA - Catalog

• Isoelectric Focusing

• SDS PAGE

• 2D Electrophoresis

• Native Electrophoresis

• Western Blotting

• Nucleic Acid Electrophoresis

• Equipment

SERVA Electrophoresis Catalog (PDF)
Close window
Watch the SERVA Video!

We are SERVA! Watch to see where we are located and how we are serving scientists worldwide.

SERVA Video

Close window
Certificates of Analysis
now online!

How to find the required certificate of analysis for your product:
In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02.
Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow.
Now select the lot number from the list and download the PDF with a click. Done.

Close window
SERVA - now on YouTube

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.