ASB-14

Synthetic detergent with a C14 alkyl tail. It is useful for solubilizing proteins for 2D-analysis. ASB-14 shows better protein solubilization properties than CHAPS, by which the identification of previously undetected membrane proteins was enabled.

Synonym: Amidosulfobetaine-14; 3-[N,N-Dimethyl-(3-myristoylaminopropyl)-ammonio]-propanesulfonate; 3-[N,N-Dimethyl-N-(3-myristamidopropyl)-ammonio)]-propane-1-sulfonate.

CAS registry number: [216667-08-2]

Molecular formula: C22H46N2O4S

Relative molecular mass (Mr): 434.7

Classification: Zwitterionic surfactant

Critical micellar concentration (CMC): 8 mM

 

Bibliography

Chevallet, M. et al. (1998) New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis. Electrophoresis 19, 1901-9.
Herbert, B. (1999) Advances in protein solbilization for two-dimensional electrophoresis. Electrophoresis 20, 660-3.

Carrol, J. et al. (2002) Definition of the nuclear encoded  protein composition of bovine heart mitochondrial complex I. Identification of two new subunits. J. Biol. Chem. 277, 50311-9.

Kim, H. et al. (2007) Solubilization conditions for hydrophobic membrane protein, oleosin in soybeans. Biotechnol. Bioproc. Engineering 12, 542-7.

Mujahid, S. et al. (2007) Improved solubilization of surface proteins from Listeria monocy-togenes for 2-DE. Electrophoresis 28, 3998-4007.

Cordwell, S.J. (2008) Sequential extraction of proteins by chemical reagents. In: Methods in Molecular Biology 424 (2D PAGE: Sample Preparation and Fractionation, vol.1) p. 139-46  (Totowa, NJ, USA).

Patel, N. et al. (2008) Strategies to recover proteins from ocular tissues for proteomics. Proteomics 8, 1055-70.

Zuobi-Hasona, K. a. Brady, L.J. (2008) Isolation and solubilization of cellular membrane proteins from bacteria. In: Methods in Molecular Biology 425 Sample Preparation and Fractionation, vol. 2) p. 287-93 (Totowa, NJ, USA).

Pieper, R. et al. (2009) Integral and peripheral association of proteins and protein complexes with Yersinia pestis inner and outer membranes. Proteome Science 7:5

Rabilloud, T. (2009) Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis. In: Methods in Molecular Biology 528: Membrane Proteomics, p. 259-67 (Totowa, NJ, USA).

Wimmer, M.A. et al. (2009) Membrane-associated, boron-interacting proteins isolated by boronate affinity chromatography. Plant Cell Physiol. 50, 1292-1304

Alhamdani, M.S.S. et al. (2010) Single-step procedure fort he isolation of proteins at near-native conditions from mammaliantissue for proteomic analysis on antibody microarrays. J. Proteome Res. 9, 063-71.

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