N-Dodecyl-N,N-dimethylammonio-3-propane sulfonate

The zwitterionic detergent N-Dodecyl-N,N-dimethylammonio-3-propane sulfonate is  primarily characterized by  its applicability over a wide pH range.

Sulfobetaine 3-12 is of interest for the investigation of photosystem particles and for preparation of virosomes.

N-Dodecyl-N, N-dimethylammonio-3-propane sulfonate is an effective desorbing agent in biospecific and bioselective affinity chromatography. It can also be used for the purification of active ribosomal complexes via adsorption onto cellulose membranes with subsequent surfactant-mediated desorption.

Synonym: 3-(Dodecyldimethylammonio))propane-1-sulfonate; N-Dodecylsulfobetaine; Ralufon DL; SB12; SB 3-12

CAS registry number: [14933-08-5]

Molecular formula: C17H37NO3S

Relative molecular mass (Mr): 335.6

Classification: Zwitterionic surfactant

Literature specification:
•    Critical micellar concentration (CMC): 3.3 – 3.6 mM
•    Aggregation number (Na): 55
•    Krafft point (TK): < 0 °C
•    pH 2 – 12: net charge = 0


Solubilization and Characterization of Photosystems

Dekker, J.P., Boekema, E.J., Witt, H.T. a. Roegner, M. (1988) Refined purification and further characterization of oxygen-evolving and Tris-treated Photosystem II particles from the thermophilic Cyanobacterium Synechococcus sp. Biochim. Biophys. Acta 936, 307-18.

Ford, R. C. a. Holzenburg, A. (1988) Investigation of the structure of trimeric and monomeric photosystem I reaction center complexes. EMBO J. 7, 2287-93.

van de Meent, E.J. et al. (1990) Purification and properties of an antenna-reaction center complex from heliobacteria. Biochim. Biophys. Acta - Bioenergetics 1015, 223-30.
Chromatographic Separations

Nollstadt, KH., Karkhanis, Y.D. Gnozzio, M.J. Crane. M.S.J., Gurnett, A.M., Schmatz, D.M. a. Turner, M.J. (1989) Potential of the sulfobetaine detergent Zwittergent 3-12 as a desorbing agent in biospecific and bioselective affinity chromatography. J. Chromatogr. 497, 87-100.

Hettmann, T. et al. (1998) Cytochrome b558/566 from the archaeon Sulfolobus avidocaldarius. A novel highly glycosylated membrane-bound b-type hemoprotein. J. Biol. Chem. 273, 12032-40.
Further Applications

Mazhul, L.A., Smirnova, G. V., Lotte, V.D.    Zhigis, L.S., Reshetov, P.D., Ageeva, O.N. a. Andzhaparidze, O.G. (1988). Use of the zwittergent sulfobetaine-12 for the preparation of virosomes and investigation of their immunogenic and protective properties. Mol. Genet., Mikrobiol. Virusol. (12), 42-7.

Theocharis, D.A. a. Coutsogeorgopoulos, C. (1989) Recovery of active ribosomal complexes from cellulose nitrate membranes. Anal. Biochem. 176, 278-83.
Sedzik, J. a. Tsukihara, T. (2000) Solubilization of PNS myelin membrane proteins by detergents. Neuroreport 11, 2559-63.

Wydro, P. a. Paluch, M. (2005) A study of the interaction of dodecyl sulfobetain with cationic and anionic surfactant in mixed micelles and monolayers at the air/water interface. J. Colloid Interface Sci. 286, 387-91.

Wydro, P. (2007) The influence of the size of the hydrophilic group on the miscibility of zwitterionic and nonionic surfactants in mixed monolayers and micelles. J. Colloid Interface Sci. 316, 107-13.


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