Tank blotting

Tank Blotting

Transfer buffer
(10x concentrate):

0.25 M Tris
1.92 M Glycin

Working solution: Dilute 100 ml of 10x concentrate with  200 ml methanol and
700 ml distilled water.

 

  1. Cut transfer membrane and four pieces Whatmann 3 MM paper to gel size (7 x 8 cm).

  2. Equilibrate the membrane in transfer buffer (Towbin buffer, cat. no. 42558). By use of PVDF membranes equilibrate the membrane first for 2 minutes in methanol and then for additional 5 minutes in transfer buffer.

  3. Wet the porous pads as well as the four pieces Whatmann 3 MM paper with transfer buffer.

  4. Remove the gel from the cassette and equilibrate the gel for 5 minutes in transfer buffer.
  5. Mount the transfer sandwich according to the manufacture’s protocol and place it in the tank blotter.

  6. Transfer is done at room temperature at 250 mA resp. ca. 60 V for ca. 1 - 2 hours.


After blotting proteins can be stained on the membrane:

  • Detection with Ponceau S solution (0.2 %, cat. no. 33427)
  • Staining with Amido black

Top
Close window
SERVA Market Square 2021: April - June

Electrophoresis:

• Vertical Protein Electrophoresis
• Horizontal Protein Electrophoresis
• Western Blotting
• Electrophoresis Reagents
• DNA/RNA Electrophoresis

Life Sciences:

• Fluorescence Labelling
• Albumins
• Protease and Phosphagtase Inhibitors
• Enzymes & Reagents for Mass Spectrometry
• Enzymes for Life Sciences

 

→ This way to the Special Offer

Close window
Product of the Month May:
Yeast Extract SERVABACTER® &
Agar Agar SERVA

Yeast Extract SERVABACTER® &
Agar Agar SERVA - High Quality Supplements for Microbiology

→ This way to the Special Offer

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.