Semi-dry protein transfer

Semi-Dry Protein Transfer

  • Cut the transfer membrane and four sheets of Whatmann 3 MM paper on gel size (7 x 8 cm).

  • Equilibrate the membrane in transfer buffer (Towbin buffer, cat. no. 42558) or, if PVDF membranes are used, first in ethanol for 2 minutes and then in transfer buffer for another 5 minutes.

  • Also moisten the four sheets of Whatmann 3 MM paper with transfer buffer.

  • Remove the gel from the cassette and equilibrate the gel in transfer buffer for 5 minutes.

  • Build the transfer sandwich analogously according to the manufacturer's instructions for the blotter and place it in the semi-dry blotter.

  • The transfer takes place at room temperature with 1.5 mA / cm2 gel area for about 1 hour (for standard marker proteins).

 

When transferring proteins of different sizes, it is advisable to use a discontinuous blotting buffer system (SERVA Semi-Dry Blotting Kit, cat no. 42559.01).


Top
Close window
SERVA Market Square 2021: September - December

• Gel Media
• Protein & DNA Standards
• Protein Quantification
• Buffers & Buffer Solutions
• Detergents

 

→ This way to the Special Offer

Close window
Product of the Month September:
AquaSpark™ Substrate

AquaSpark™ Substrate for Chemiluminescence Detection of Alkaline Phosphatase (AP) in ELISA and Blot

→ This way to the Special Offer

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.