Amido Black staining of the transfer membrane

Amido Black Staining of transfer membranes


  • Amido Black Staining solution (10x concentrate): 
    1 % (w/v) Amido Black dissolved in 40 % (v/v) Ethanol und 10 % (v/v) Glacial acetic acid

  • Destaining solution:
    40 % (v/v) Ethanol, 10 % (v/v) Glacial acetic acid and 2 % (v/v) Glycerol


Staining protocol:

The blotting membrane is incubated for 5 minutes in amido black staining solution (1:10 dilution) and then destained in destaining solution.


Note: Amido black is not a reversible stain, but more sensitive in detection than Ponceau S, comparable to a Coomassie® Brilliant Blue R stain.

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